Journal
BIOMATERIALS
Volume 27, Issue 36, Pages 6083-6095Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2006.07.045
Keywords
tissue engineering; mesenchymal stem cell; bioreactor; flexure; flow; fluid shear stress
Funding
- NHLBI NIH HHS [HL 68816] Funding Source: Medline
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Bone marrow-derived mesenchymal stem cells (BMSCs) are relatively accessible and exhibit a pluripotency suitable for cardiovascular applications such as tissue-engineered heart valves (TEHVs). Recently, Sutherland et al. [From stem cells to viable autologous semilunar heart valve. Circulation 2005; 111(21): 2783-91] demonstrated that BMSC-seeded TEHV can successfully function as pulmonary valve substitutes in juvenile sheep for at least 8 months. Toward determining appropriate mechanical stimuli for use in BMSC-seeded TEHV cultivation, we investigated the independent and coupled effects of two mechanical stimuli physiologically relevant to heart valves-cyclic flexure and laminar flow (i.e. fluid shear stress)-on BNISC-mediated tissue formation. BMSC isolated from juvenile sheep were expanded and seeded onto rectangular strips of nonwoven 50:50 blend poly(glycolic acid) (PGA) and poly(L-lactic acid) (PLLA) scaffolds. Following 4 days static culture, BMSC-seeded scaffolds were loaded into a novel flex-stretch-flow (FSF) bioreactor and incubated under static (n = 12), cyclic flexure (n = 12), laminar flow (avg. wall shear stress = 1.1505 dyne/cm(2); 11 = 12) and combined flex-flow (n = 12) conditions for 1 (n = 6) and 3 (n = 6) weeks. By 3 weeks, the flex-flow group exhibited dramatically accelerated tissue formation compared with all other groups, including a 75% higher collagen content of 844 +/- 278 mu g/g wet weight (P < 0.05), and an effective stiffness (E) value of 948 +/- 233 kPa. Importantly, collagen and E values were not significantly different from values measured for vascular smooth muscle cell (SMC)-seeded scaffolds incubated under conditions of flexure alone [Engelmayr et al. The independent role of cyclic flexure in the early in vitro development of an engineered heart valve tissue. Biomaterials 2005; 26(2): 175-87], suggesting that BMSC-seeded TEHV can be optimized to yield results comparable to SMC-seeded TEHV. We thus demonstrated that cyclic flexure and laminar flow can synergistically accelerate BMSC-mediated tissue formation, providing a basis for the rational design of in vitro conditioning regimens for BMSC-seeded TEHV. (c) 2006 Elsevier Ltd. All rights reserved.
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