Journal
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
Volume 37, Issue 4, Pages 474-480Publisher
WILEY
DOI: 10.1111/j.1749-7345.2006.00060.x
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To examine the need for supplemental dietary methionine and to determine the ability of different methionine sources to meet this need for Pacific white shrimp, Litopenaeus vannamei, a growth trial was conducted. A control diet (30% crude protein) was formulated to be deficient in methionine by inclusion of high levels of dehulled, solvent-extracted soybean meal and with no fish meal (methionine level was 0.45% of diet as fed, 1.5% of total amino acids). Three test diets were manufactured based on the control diet but supplemented with L-methionine, DL-methionine, or an analog of methionine (2-hydroxy-4-methylthio butanoic acid [HMTBA], calcium salt) at the level of 0.5% as fed (equivalent to 1.5% of total amino acids). Cystine was constant among these diets at 0.5% of diet as fed. A commercial feed (30% crude protein) was included as a reference. The culture vessels were flat-bottomed, cylindrical, black fiberglass tanks (1.5-m diameter) initially filled with 1000 L seawater and supplied with aeration and seawater from a well at a rate of 2 L/min. At the end of the trial, all surviving shrimp were counted and weighed. The trial was run outdoors, with a mean water temperature of 25.9 C. The mean survival, final weight, growth rate, and feed efficiency of shrimp in the control group were 95.9%, 10.8 g, 0.93 g/wk, and 0.44 g/g, respectively. The mean values of all the test treatments were 96.9%, 11.6 g, 1.00 g/wk, and 0.47 g/g, respectively. The final weight and growth rate of shrimp fed the control diet were significantly lower than those of shrimp fed the methionine-supplemented diets, indicating that this species has a requirement for dietary methionine. There were no significant differences (P > 0.05) in any of the parameters among the test treatments, indicating that L-methionine, DL-methionine, and HMTBA can be used to meet the methionine requirement of this species under these experimental conditions. It is concluded that the three sources of methionine examined in this trial, L-methionine, DL-methionine, and HMTBA, are similar in their ability to meet the methionine requirement of this species.
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