Journal
NATURE GENETICS
Volume 38, Issue 12, Pages 1406-1412Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ng1906
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Funding
- NIGMS NIH HHS [GM62791, 1F32GM074590-01] Funding Source: Medline
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We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We identified 13 different de novo mutations in five different E. coli strains and monitored their fixation over a 44-d period of adaptation. We obtained proof that the observed spontaneous mutations were responsible for improved fitness by creating single, double and triple site-directed mutants that had growth rates matching those of the evolved strains. The success of this new genome-scale approach indicates that real-time evolution studies will now be practical in a wide variety of contexts.
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