Journal
JOURNAL OF PROTEOME RESEARCH
Volume 5, Issue 12, Pages 3459-3469Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr060403j
Keywords
plant mitochondria; Arabidopsis; ATP affinity; ATP synthesis; respiration
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The interactions of ATP inside plant mitochondria were investigated by identifying the soluble nucleotide binding proteome captured using immobilized ATP. Selected proteins were separated by 1D SDS-PAGE and 2D IEF-SDS-PAGE and identified by ESI-Q-TOF MS/MS. A range of highly enriched proteins were identified from the mitochondrial proteome, including 14-3-3 proteins and RNA binding proteins, as well as proteins known to contain nucleotide binding domains and/or to be inhibited or stimulated by ATP.
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