Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 99, Issue 5, Pages 1285-1297Publisher
WILEY
DOI: 10.1002/jcb.20904
Keywords
mesenchymal stem cells; bone marrow; adipose tissue; differentiation; telomerase; transcription factors
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Funding
- NCRR NIH HHS [P51 RR000164, RR00164] Funding Source: Medline
- NIDDK NIH HHS [P30 DK072476] Funding Source: Medline
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The biologic characteristics of mesenchymal stem cells (MSCs) isolated from two distinct tissues, bone marrow and adipose tissue were evaluated in these studies. MSCs derived from human and non-human primate (rhesus monkey) tissue sources were compared. The data indicate that MSCs isolated from rhesus bone marrow (rBMSCs) and human adipose tissue (hASCs) had more similar biologic properties than MSCs of rhesus adiposetissue (rASCs) and human bone marrow MSCs (hBMSCs). Analyses of in vitro growth kinetics revealed shorter doubling time for rBMSCs and hASCs. rBMSCs and hASCs underwent significantly more population doublings than the other MSCs. MSCs from all sources showed a marked decrease in telomerase activity over extended culture; however, they maintained their mean telomere length. All of the MSCs expressed embryonic stem cell markers, Oct-4, Rex-1, and Sox-2 for at least 10 passages. Early populations of MSCs types showed similar multilineage differentiation capability. However, only the rBMSCs and hASCs retain greater differentiation efficiency at higher passages. Overall in vitro characterization of MSCs from these two species and tissue sources revealed a high level of common biologic properties. However, the results demonstrate clear biologic distinctions, as well.
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