Journal
JOURNAL OF CHEMICAL ECOLOGY
Volume 32, Issue 12, Pages 2679-2685Publisher
SPRINGER
DOI: 10.1007/s10886-006-9191-z
Keywords
abietic; neoabietic; palustric; levopimaric; dehydroabietic; white spruce; diterpenes; HPLC
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Diterpene resin acids are major constituents of conifer oleoresin and play important roles in tree defense against insects and microbial pathogens. The tricyclic C-20 carboxylic acids are generally classified into two groups, the abietanes and the pimaranes. The abietanes have conjugated double bonds and exhibit characteristic UV spectra. Here, we report the analysis of abietanes by reversed-phase high-performance liquid chromatography using multiwavelength detection to optimize quantification of underivatized abietic, neoabietic, palustric, levopimaric, and dehydroabietic acids. The utility of the method is demonstrated with methanol extracts of white spruce (Picea glauca) phloem, and representative concentrations are reported.
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