Journal
ULTRASOUND IN MEDICINE AND BIOLOGY
Volume 32, Issue 12, Pages 1931-1941Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ultrasmedbio.2006.06.020
Keywords
ultrasound; cavitation; Levovist (R); sonoporation; inertial cavitation dose; HeLa
Ask authors/readers for more resources
Ultrasound-induced acoustic cavitation assists gene delivery, possibly by increasing the permeability of the cell membranes. How the cavitation dose is related to the sonoporation rate and the cell viability is still unknown and so this in vitro study quantitatively investigated the effects of cavitation induced by 1-MHz pulsed ultrasound waves and the contrast agent Levovist((R)) (containing microbubbles when reconstituted by adding saline and shaken) on the delivery of short DNA-FITC molecules into HeLa cells. The concentrations of cells and DNA-FITC were 2 X 10(5) cells/mL and 40 mu g/mL, respectively. The cavitation was quantified as the inertial cavitation dose (ICD), corresponding to the spectral broadband signal enhancement during microbubble destruction. The relations of ICD with sonoporation and cell viability were examined for various acoustic pressures (0.48-1.32 MPa), Levovist((R)) concentrations (1.12 x 10(5)-1.12 X 10(7) bubbles/mL) and pulse durations (1-10 cycles). The linear regressions of the sonoporation rate versus ICD and the cell viability versus ICD were y = 28.67x + 10.71 (R-2 = 0.95) and z = -62.83x + 91.18 (R-2 = 0.84), respectively, where x is ICD, y is the sonoporation rate and Z is the cell viability. These results show that the sonoporation rate and the cell viability are highly correlated with the ICD, indicating that sonoporation results may be potentially predicted using ICD.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available