Journal
NATURE BIOTECHNOLOGY
Volume 24, Issue 12, Pages 1569-1571Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt1264
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Funding
- NIBIB NIH HHS [EB-000205] Funding Source: Medline
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The utility of blue fluorescent protein ( BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield ( 0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.
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