Journal
CONSERVATION GENETICS
Volume 7, Issue 6, Pages 1001-1003Publisher
SPRINGER
DOI: 10.1007/s10592-006-9120-2
Keywords
microsatellite DNA genotyping; non-invasive sampling; Sphenodon; tuatara
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In this study, a minimally invasive method for DNA sampling of reptiles and amphibians using cloacal and buccal swabs is described. High molecular weight DNA was isolated from the swabs, which were collected from tuatara (Sphenodon punctatus), and stored in 70% ethanol at room temperature for approximately 1 week. Amplification of mitochondrial and microsatellite DNA loci was successful from both cloacal and buccal swabs, and in all cases the genotypes matched those obtained from blood samples. These results show that cloacal and/or buccal swabbing is a useful alternative to blood sampling and toe clipping for genetic studies on reptiles. This method is rapid, inexpensive and easy to implement in field situations.
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