Journal
PROTEOMICS
Volume 6, Issue 24, Pages 6549-6553Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200600191
Keywords
cartilage; extracellular matrix proteins; IEF optimization; prefractionation
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Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.
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