Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 18, Issue 6, Pages 769-781Publisher
SPRINGER
DOI: 10.1007/s10811-006-9088-9
Keywords
diatom; Haslea ostrearia; microalgae; marennine; pigment; ultrafiltration
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The diatom Haslea ostrearia that lives in oyster ponds has the distinctive feature of synthesizing marennine, a blue-green pigment of which the chemical nature still remains unknown. This pigment is responsible for the greening of oyster gills. Here, we report a new method for extraction and purification of intracellular (accumulated in the apex of the cell) and extracellular (released into the external medium) forms of the pigment. Intracellular marennine is obtained by extraction from blue algal pellets with a carbonate buffer. The extract is then centrifuged and filtered. Extracellular marennine is obtained by clarification of blue-coloured culture medium. Both extracts are then purified by a semi-preparative process, using ultrafiltration through membranes and anion-exchange chromatography. This procedure allows us to produce native pigment displaying the degree of purity required to enter upon the molecular characterisation of marennine. By this process, about 35% of the initial amount of pigment can be recovered. If necessary, this method could be easily scaled up to a larger production system to accommodate potential industrial applications.
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