Journal
JOURNAL OF IMMUNOLOGY
Volume 177, Issue 11, Pages 7707-7714Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.177.11.7707
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Funding
- NCI NIH HHS [R01 CA 85862-06] Funding Source: Medline
- NIA NIH HHS [T32 AG 20013] Funding Source: Medline
- NIGMS NIH HHS [T32 GM008692, T32 GM 07215-25] Funding Source: Medline
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The leukocyte integrin LFA-1 plays a critical role in T cell trafficking and T cell adhesion to APCs. It is known that integrin-mediated adhesion is regulated by changes in integrin ligand-binding affinity and valency through inside-out signaling. However, the molecular mechanisms involved in TCR-mediated LFA-1 regulation are not well understood. In this study, we show that the cytoskeletal protein talin1 is required for TCR-mediated activation of LFA-1 through regulation of LFA-1 affinity and clustering. Depletion of talin1 from human T cells by small interfering RNAs impairs TCR-induced adhesion to ICAM-1 and T cell-APC conjugation. TCR-induced LFA-1 polarization, but not actin polarization, is defective in talin1-deficient T cells. Although LFA-1 affinity is also reduced in talin1-deficient T cells, rescue of LFA-1 affinity alone is not sufficient to restore LFA-1 adhesive function. Together, our findings indicate that TCR-induced up-regulation of LFA-1-dependent adhesiveness and resulting T cell-APC conjugation require talin1.
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