Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 20, Issue 12, Pages 1309-1314Publisher
WILEY
DOI: 10.1002/bmc.697
Keywords
clopidogrel; antiplatelet; carboxylic acid metabolite; reversed-phase chromatography
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A new, simple, and reproducible method for determination of carboxylic acid metabolite of clopiclogrel in human plasma has been developed. After liquid-liquid extraction in acidic medium with chloroform, samples were quantified on a Nova-pak C-8 5 mu m column using a mixture of 30 mm K2HPO4-THF-acetonitrile (pH=3, 79:2:19, v/v/v) as mobile phase with UV detection at 220 nm. The flow rate was set at 0.9 mL/min. Ticlopicline was used as internal standard and the total run time of analysis was about 12 min. The method was linear over the range of 0.2-10 mu g/mL of clopiclogrel metabolite in plasma (r(2) > 0.999). The within-day and between-day precision values were in the range 1.0-4.8%. The limit of quantification of the method was 0.2 mu g/mL. The method was successfully used to study the pharmacokinetics of clopiclogrel in healthy volunteers. Copyright (c) 2006 John Wiley & Sons, Ltd.
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