Journal
FEMS MICROBIOLOGY LETTERS
Volume 265, Issue 2, Pages 249-255Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1574-6968.2006.00503.x
Keywords
Escherichia coli; natural transformation; static culture
Categories
Ask authors/readers for more resources
A novel transformation system, in which neither a nonphysiological concentration of Ca2+ and temperature shifts nor electronic shocks were required, was developed to determine whether Escherichia coli is naturally transformable. In the new protocol, E. coli was cultured normally to the stationary phase and then cultured statically at 37 degrees C in Luria-Bertani broth. After static culture, transformation occurred in bacteria spread on Luria-Bertani plates. The protein synthesis inhibitor chloramphenicol inhibited this transformation process. The need for protein synthesis in plated bacteria suggests that the transformation of E. coli in this new system is regulated physiologically.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available