Journal
PROCESS BIOCHEMISTRY
Volume 41, Issue 12, Pages 2424-2428Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2006.06.030
Keywords
feedback control; ethanol concentration; high-cell-density culture; amino acid modulation; glutathione production; Saccharomyces cerevisiae
Ask authors/readers for more resources
In this paper the high-cell-density fed-batch culture and optimal amino acid modulation were combined together to enhance glutathione production in Saccharomyces cerevisiae T65. Ethanol concentration in the broth was an important parameter for feedback control in fed-batch culture. Low ethanol concentration was propitious to both the cell growth and glutathione synthesis. The feedback control of a low ethanol concentration was an efficient way to realize high-cell-density culture and the biomass reached 140 g/L after 57 h fermentation. With optimal amino acid addition to elevate the glutathione content continually, the maximum glutathione yield achieved 2190 mg/L. (c) 2006 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available