Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 103, Issue 49, Pages 18493-18498Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0604977103
Keywords
deuterium exchange; dynamics; solvent; hydrogen; crystallography
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Hydrogen atoms play a central role in many biochemical processes yet are difficult to visualize by x-ray crystallography. Spallation neutron sources provide a new arena for protein crystallography with TOF measurements enhancing data collection efficiency and allowing hydrogen atoms to be located in smaller crystals of larger biological macromolecules. Here we report a 2.2-angstrom resolution neutron structure of Escherichia coli dihydrofolate reductase (DHFR) in complex with methotrexate (MTX). Neutron data were collected on a 0.3-mm(3) D2O-soaked crystal at the Los Alamos Neutron Scattering Center. This study provides an example of using spallation neutrons to study protein dynamics, to identify protonation states directly from nuclear density maps, and to analyze solvent structure. Our structure reveals that the occluded loop conformation [monomer (mon.) A] of the (DHFRMTX)-M-. complex undergoes greater H/D exchange compared with the closed-loop conformer (mon. B), partly because the Met-20 and 13(F-G) loops readily exchange in mon. A. The eight-stranded beta sheet of both DHFR molecules resists H/D exchange more than the helices and loops. However, the C-terminal strand, beta H, in mon. A is almost fully exchanged. Several D2OS form hydrogen bonds with exchanged amides. At the active site, the N1 atom of MTX is protonated and thus charged when bound to DHFR. Several D2Os are observed at hydrophobic surfaces, including two pockets near the MTX-binding site. A previously unidentified D2O hydrogen bonds with the catalytic D27 in mon. B, stabilizing its negative charge.
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