Journal
BRAIN RESEARCH BULLETIN
Volume 71, Issue 1-3, Pages 23-28Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2006.07.011
Keywords
migraine aura; gap junctions; connexin 36; MK-801
Categories
Ask authors/readers for more resources
A protocol for inducing cortical spreading depression (SD) on rat neocortical slices in vitro, upon local application of calibrated similar to nl drops of KCI, 3M was used to elicit SD events, recorded at two different points on the slice. This in vitro model was validated by the inhibition of SD episodes by the NMDA antagonist MK-801 (20 mu M), the reference SD blocker. Quinine, its stereoisomer quinidine, and mefloquine consistently inhibited the SD episodes. Quinine and quinidine, 100 and 200 mu M reduced the duration, while mefloquine, 100 and 200 mu M reduced the amplitude of SD events, all in a concentration-dependent manner. These compounds have been reported to block gap junctions, specifically the neuronal connexin (Cx) 36, but they also exert other cellular effects. While further investigation is warranted to settle whether SID inhibition in vitro by quinine, quinidine and mefloquine reflects an involvement of neuronal Cx36 channels in SD generation/propagation, these results bear potential drug-discovery relevance for the migraine with aura. (c) 2006 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available