Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 351, Issue 2, Pages 528-533Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2006.10.060
Keywords
Mycobacterium leprae; truncated hemoglobin; GlbO; peroxynitrite; carbon dioxide; scavenging of nitrogen reactive species
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Mycobacterium leprae GlbO has been proposed to represent merging of both O-2 uptake/transport and scavenging of nitrogen reactive species. Peroxynitrite reacts with M. leprae GlbO(II)-NO leading to GlbO(III) via the GlbO(III)-NO species. The value of the second order rate constant for GlbO(III)-NO formation is > 1 x 10(8) M-1 s(-1) in the absence and presence of CO2 (1.2 x 10(-3) M). The CO2-independent value of the first order rate constant for GlbO(III)-NO denitrosylation is (2.5 +/- 0.4) x 10(1) s(-1). Furthermore, peroxynitrite reacts with GlbO(II)O-2 leading to GlbO(III) via the GlbO(IV)=O species. Values of the second order rate constant for GlbO(IV)=O formation are (4.8 +/- 0.5) x 10(4) and (6.3 +/- 0.7) x 10(1) M-1 s(-1) in the absence and presence of CO2 (=1.2 x 10(-3) M), respectively. The value of the second order rate constant for the peroxynitrite-mediated GlbO(IV)=O reduction (=(1.5 +/- 0.2) x 10(4) M-1 S-1) is CO2-independent. These data argue for a role of GlbO in the defense of M. leprae against nitrosative stress. (c) 2006 Elsevier Inc. All rights reserved.
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