Journal
ANALYTICAL CHEMISTRY
Volume 78, Issue 24, Pages 8313-8318Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0613582
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Funding
- NIH HHS [DP1 OD000285, DP1 OD000285-02] Funding Source: Medline
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We have investigated the variables that influence DNA coverage on gold nanoparticles. The effects of salt concentration, spacer composition, nanoparticle size, and degree of sonication have been evaluated. Maximum loading was obtained by salt aging the nanoparticles to similar to 0.7 M NaCl in the presence of DNA containing a poly( ethylene glycol) spacer. In addition, DNA loading was substantially increased by sonicating the nanoparticles during the surface loading process. Last, nanoparticles up to 250 nm in diameter were found have similar to 2 orders of magnitude higher DNA loading than smaller (13-30 nm) nanoparticles, a consequence of their larger surface area. Stable large particles are attractive for a variety of biodiagnostic assays.
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