Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 351, Issue 3, Pages 756-763Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2006.10.105
Keywords
micro RNA (miRNA); Piwi-interacting RNA (piRNA); small nucleolar RNAs (snoRNAs); real-time quantitative PCR (Q-PCR); small RNA cDNA (srcDNA)
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Funding
- NICHD NIH HHS [R03 HD048855, HD048855] Funding Source: Medline
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Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method. (c) 2006 Elsevier Inc. All rights reserved.
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