Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1137, Issue 1, Pages 22-29Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2006.09.082
Keywords
quinolones; plasma analysis; solid-phase extraction; liquid chromatography; mass spectrometry
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A simple and sensitive liquid chromatography (LC) method was developed for the simultaneous determination of eight quinolones in pig plasma samples. The following two methods of detection were used: ultraviolet (UV) and mass spectrometry with electrospray ionization (ESI/MS). Sample preparation consisted of solid-phase extraction (SPE) on Strata X cartridges prior to the analysis by LC/UV or LC/ESI/MS. The recovery, linearity, limit of detection (LOD) and limit of quantification (LOQ), precision and accuracy of the method were evaluated using spiked pig plasma samples. The suitability of the method for pharmacokinetic studies was evaluated by determining the concentrations of enrofloxacin (ENR) and ciprofloxacin (CIP) also in pig plasma, after administration of 200 mg of enrofloxacin per kilogram of fodder during 5 consecutive days. (c) 2006 Elsevier B.V. All rights reserved.
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