Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 553, Issue 1-3, Pages 120-128Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2006.09.047
Keywords
GABA(A) receptors; IPSC; substantia gelatinosa; propofol; midazolam; patch clamp; (mouse)
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The physiological and pharmacological properties of gamma-aminobutyric acid (GABA)ergic miniature inhibitory postsynaptic currents (mIPSCs) were investigated in substantia gelatinosa neurons of mouse spinal cord using whole-cell patch clamp recordings. Two cell populations were pharmacologically identified based on the effect of propofol (10 mu M) on the mIPSC decay kinetics: those exhibiting propofol-sensitive mIPSCs, with a slow decay kinetic (mIPSC(SLOW)), and those exhibiting propofol-resistant mIPSCs, with a fast decay kinetic (mIPSC(FAST)) (decay time constants of 14.2 +/- 0.7 and 7.4 +/- 0.8 ins, respectively). The frequency and amplitude of both types of mIPSCs were not affected by propofol. Miniature IPSCFAST showed midazolam insensitivity, while midazolam prolonged the decay phase of mIPSC(SLOW) without modulation of the frequency and amplitude. Exogenous GABA-evoked responses in the neurons with mIPSC(SLOW) were potentiated by propofol, while those in neurons with mIPSC(FAST) were unaffected by propofol. Furthermore, non-stationary noise analysis of the two kinetically and pharmacologically distinct mIPSCs revealed different conductance of GABA(A) receptor channels underlying the synaptic events. Pharmacological responses to propofol and midazolam suggested that mIPSC(FAST) and mIPSC(SLOW) in substantia gelatinosa neurons can be mediated by GAB(A) receptors with different subunit compositions. (c) 2006 Elsevier B.V. All rights reserved.
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