Journal
ADVANCED DRUG DELIVERY REVIEWS
Volume 58, Issue 15, Pages 1622-1654Publisher
ELSEVIER
DOI: 10.1016/j.addr.2006.09.018
Keywords
combinatorial peptide libraries; vascular cell surface proteomics; targetomics; targeted therapies; display scaffold; phage display; viral display; bacterial display; yeast display; cell display; ribosome display; mRNA display; covalent DNA display
Categories
Funding
- NCI NIH HHS [R01 CA088106, P50 CA100632, P30 CA016672, P01 CA049639, R01 CA078512, R01 CA081247, R33 CA103056, R33 CA103030, R21 CA111853, R01 CA085843] Funding Source: Medline
- NHLBI NIH HHS [R01 HL081658] Funding Source: Medline
- NIDDK NIH HHS [R01 DK067683] Funding Source: Medline
Ask authors/readers for more resources
Recognition of molecular diversity of cell surface proteornes in disease is essential for the development of targeted therapies. Progress in targeted therapeutics requires establishing effective approaches for high-throughput identification of agents specific for clinically relevant cell surface markers. Over the past decade, a number of platform strategies have been developed to screen polypeptide libraries for ligands targeting receptors selectively expressed in the context of various cell surface proteomes. Streamlined procedures for identification of ligand-receptor pairs that could serve as targets in disease diagnosis, profiling, imaging and therapy have relied on the display technologies, in which polypeptides with desired binding profiles can be serially selected, in a process called biopanning, based on their physical linkage with the encoding nucleic acid. These technologies include virus/phage display, cell display, ribosomal display, mRNA display and covalent DNA display (CDT), with phage display being by far the most utilized. The scope of this review is the recent advancements in the display technologies with a particular emphasis on molecular mapping of cell surface proteomes with peptide phage display. Prospective applications of targeted compounds derived from display libraries in the discovery of targeted drugs and gene therapy vectors are discussed. (c) 2006 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available