Journal
EXPERIMENTAL AND MOLECULAR MEDICINE
Volume 38, Issue 6, Pages 599-605Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/emm.2006.71
Keywords
adipocytes; berberine; inflammation; lipoprotein lipase; PPAR-gamma; tumor necrosis factor-alpha
Ask authors/readers for more resources
Berberine (BBR), an isoquinoline alkaloid, has a wide range of pharmacological effects, yet its exact mechanism is unknown. In order to understand the anti-adipogenic effect of BBR, we studied the change of expression of several adipogenic enzymes of 3T3-L1 cells by BBR treatment. First, we measured the change of leptin and glycerol in the medium of 3T3-L1 cells treated with 1 mu M, 5 mu M and 10 mu M concentrations of BBR. We also measured the changes of adipogenic and lipolytic factors of 373-L1. In 3T3-L1 cells, both leptin and adipogenic factors (SREBP-1c, CIEBP-alpha, PPAR-gamma, fatty acid synthase, acetyl-CoA carboxylase, acyl-CoA synthase and lipoprotein lipase) were reduced by BBR treatment. Glycerol secretion was increased, whereas expression of lipolytic enzymes (hormone-sensitive lipase and perilipin) mRNA was slightly decreased. Next, we measured the change of inflammation markers of 3T3-L1 cells by BBR treatment. This resulted in the down-regulation of mRNA level of inflammation markers such as TNF-alpha, IL-6, C-reactive protein and haptoglobin. Taken together, our data shows that BBR has both anti-adipogenic and anti-inflammatory effects on 3T3-L1 adipocytes, and the anti-adipogenic effect seems to be due to the down-regulation of adipogenic enzymes and transcription factors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available