Journal
ANIMAL REPRODUCTION SCIENCE
Volume 119, Issue 3-4, Pages 275-281Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.anireprosci.2010.01.003
Keywords
Recombinant gonadotropin releasing hormone; Boars; Immunological castration
Funding
- Natural Science Foundation of Anhui Education Department [KJ2007B175]
- Tianjin Municipal Science and Technology Commission [08JCYBJC27400]
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Immunization using recombinant maltose binding protein-gonadotropin releasing hormone (MBP-GnRH6) altered both testicular development and transcription of the pituitary GnRH receptor (GnRHR) gene in boars. Scrotal measurement and blood samples were taken at 4-week interval after immunization at 9 weeks of age. The concentrations of testosterone and anti-GnRH antibodies in serum were determined by radioimmunoassay and enzyme-linked immunosorbent assay, respectively. The results showed that active immunization with MBP-GnRH6 increased the serum concentration of anti-GnRH antibodies (P < 0.05) and reduced the serum concentration of testosterone (P < 0.05) as compared with MBP controls. At 25 weeks of age, boars were sacrificed and testes were evaluated histologically. Testicular development was suppressed in the MBP-GnRH6 immunized animals as compared with MBP immunized boars. MBP-GnRH6 immunized pigs exhibited mounting behavior 4 weeks later than MBP immunized boars. No mature spermatozoa were observed from MBP-GnRH6 immunized animals. By real-time quantitative PCR analysis, the amount of GnRHR mRNA in the pituitary tissue was found to be significantly lower in MBP-GnRH6 immunized animals than in controls (P < 0.05). These data demonstrate that recombinant MBP-GnRH6 was effective in immunological castration in boars. (C) 2010 Elsevier B.V. All rights reserved.
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