Journal
ANIMAL REPRODUCTION SCIENCE
Volume 118, Issue 2-4, Pages 255-264Publisher
ELSEVIER
DOI: 10.1016/j.anireprosci.2009.08.001
Keywords
Cholesterol; Semen; Equine; Cryopreservation; Acrosome reaction; Fertility
Funding
- American Quarter Horse Association
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Irreversible damage occurs to spermatozoal membranes, during the phase transition, when spermatozoa are cooled from room temperature to 5 degrees C. Some of this damage can be ameliorated by adding cholesterol to the membrane, thereby altering membrane lipid composition. Adding cholesterol-loaded cyclodextrins (CLCs) to stallion spermatozoa prior to freezing, increases cell cryosurvival. However, the fertilizing potential of CLC-treated stallion spermatozoa is unknown. To address this, experiments were conducted which evaluated the ability of CLC-treated stallion spermatozoa to capacitate, acrosome react, and bind to the zona pellucida in vitro, and to fertilize oocytes in vivo. When CLC-treated cryopreserved stallion spermatozoa were treated with various agents to induce capacitation and the acrosome reaction (AR), dilauroylphosphatidylcholine (PC-12) and lysophosphatidylcholine (LPC) induced the AR in control cells (62% and 55%, respectively) but not in CLC-treated cells (17% and 14%. respectively, P<0.05). However, the calcium ionophore A23187 induced the AR in both control- and CLC-treated cells equally well (39%, P>0.05). Control- and CLC-treated stallion spermatozoa bound to ZP of cattle oocytes equally well (0.44 +/- 0.16 vs. 0.25 +/- 0.09, respectively; P>0.05). In addition, the fertility rates of mares inseminated with control- and CLC-treated sperm were similar (P>0.05). (C) 2009 Elsevier B.V. All rights reserved.
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