Journal
NATURE PROTOCOLS
Volume 2, Issue 12, Pages 3153-3165Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2007.471
Keywords
-
Categories
Funding
- NHLBI NIH HHS [R43 HL072539, R43 HL072539-01] Funding Source: Medline
- NICHD NIH HHS [P01 HD032652-100006, P01 HD032652, P01 HD032652-09A10006, P01 HD032652-110006, P01 HD032652-120006, P01 HD032652-130006] Funding Source: Medline
Ask authors/readers for more resources
Nonviral, DNA-mediated gene transfer is an alternative to viral delivery systems for expressing new genes in cells and tissues. The Sleeping Beauty (SB) transposon system combines the advantages of viruses and naked DNA molecules for gene therapy purposes; however, efficacious delivery of DNA molecules to animal tissues can still be problematic. Here we describe the hydrodynamic delivery procedure for the SB transposon system that allows efficient delivery to the liver in the mouse. The procedure involves rapid, high-pressure injection of a DNA solution into the tail vein. The overall procedure takes <1 h although the delivery into one mouse requires only a few seconds. Successful injections result in expression of the transgene in 5-40% of hepatocytes 1 d after injection. Several weeks after injection, transgene expression stabilizes at similar to 1% of the level at 24 h, presumably owing to integration of the transposons into chromosomes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available