Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 31, Issue 1, Pages 52-60Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.dci.2006.04.001
Keywords
chicken; cytokines; immunology; genetics; Salmonella enteritidis; spleen; cecum
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We examined mRNA expression of 11 genes: BAK, Bcl-x, Interferon [IFN]-gamma, Interleukin [IL]-1 beta, IL-6, IL-10, IL-12 alpha, IL-12 beta, IL-18, CXCLi2 [IL-8/CAF], and a MIP family chemokine, CCLi2, in the spleen and cecum of day-old chicks after oral inoculation with Salmonella enteritidis (SE) or medium. Three distinct chicken breeds (broiler, Fayoumi, and Leghorn) were evaluated for mRNA expression levels at 2 and 18 h post-inoculation using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). SE exposure significantly increased splenic IL-18 and IFN-gamma expression. Breed effect was significant (P < 0.05) for CXCLi2, IL-10, IL-12 alpha, and CCLi2 mRNA expression in the spleen, and for IL-12 alpha, IL-12 beta, IL-18, and CCLi2 mRNA expression in the cecum. Generally, mRNA expression levels were higher in the spleen, and lower in the cecum, of Leghorns versus broilers. These results support a role for breed genetics influencing cytokine mRNA expression in young chickens and may potentially explain some generalized immune response differences between breeds. (c) 2006 Elsevier Ltd. All rights reserved.
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