Journal
EUROPEAN JOURNAL OF ENTOMOLOGY
Volume 104, Issue 1, Pages 81-87Publisher
CZECH ACAD SCI, INST ENTOMOLOGY
DOI: 10.14411/eje.2007.013
Keywords
Cecidomyiidae; Aphidoletes aphidimyza; biological control; aphids; insect embryogenesis; embryonic markers; cryopreservation of eggs; entomophagous insects
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Determination of embryonic stages is an important prerequisite for the long-term cryopreservation of eggs and embryos of the predatory gall midge Aphidoletes aphidimyza. This paper describes the embryonic development of this insect based on light microscopy. Gall midge embryogenesis lasts, on average, 102 h at 17 degrees C and 144 h at 15 degrees C. Living embryos can be quickly separated into ten stages that are clearly defined by specific morphological markers. The necessity for selecting definite embryonic stages for cryobiological storage is discussed.
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