Journal
SCIENTIA HORTICULTURAE
Volume 111, Issue 2, Pages 140-145Publisher
ELSEVIER
DOI: 10.1016/j.scienta.2006.10.018
Keywords
clones; genetic diversity; genetic relationships; microsatellites; 'Miraflores' peach variety; traditional peach varieties
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Some traditional peach varieties, originated from the region of Aragon (Spain), were analysed by SSRs (simple sequence repeats). The aim of this research was to characterize 19 clones related to Miraflores variety, with unknown pedigrees, to assess their genetic diversity and to elucidate their possible relationships with 10 traditional peach varieties. Twenty SSR primer pairs with high levels of polymorphism, which have been previously developed for peach, were used in this study. A total of 46 alleles were obtained for all the microsatellites studied, ranging from one to six alleles per locus, with a mean value of 2.3 alleles per locus. Fourteen SSRs were polymorphic in the set of varieties studied and permitted to distinguish 16 different genotypes out of the 30 initially studied, although fourteen 'Miraflores' clones showed identical gel profiles. The genetic distance matrix was used to construct neighbor joining cluster and to perform principal coordinate analysis which allowed the arrangement of all the genotypes according to their genetic relationships. The genetic relationships among these traditional peach varieties, and in particular among 'Miraflores' clones are discussed. The obtained results confirm that microsatellite markers are very useful for these purpose. (c) 2006 Elsevier B.V. All rights reserved.
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