Journal
JOURNAL OF BIOTECHNOLOGY
Volume 127, Issue 3, Pages 335-347Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2006.07.012
Keywords
comparative evaluation; prokaryotic expression systems; eukaryotic expression systems
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The expression of heterologous proteins in microorganisms using genetic recombination is still the high point in the development and exploitation of modem biotechnology. People can produce bioactive proteins from relatively cheap culture medium instead of expensive extraction. Host cell systems for the expression of heterologous genes are generally prokaryotic or eukaryotic systems, both of which have inherent advantages and drawbacks. An optimal expression system can be selected only if the productivity, bioactivity, purpose, and physicochemical characteristics of the interest protein are taken into consideration, together with the cost, convenience and safety of the system itself. Here, we concisely review the most frequently used prokaryotic, yeast, insect and mammalian expression systems, as well as expression in eukaryote individuals. The merits and demerits of these systems are discussed. (c) 2006 Elsevier B.V. All rights reserved.
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