Journal
JOURNAL OF CELL BIOLOGY
Volume 176, Issue 2, Pages 141-146Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200609158
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Funding
- Medical Research Council [MC_U105663148, MC_U105663140, MC_U105674181] Funding Source: researchfish
- MRC [MC_U105674181, MC_U105663148, MC_U105663140] Funding Source: UKRI
- Medical Research Council [MC_U105663140, MC_U105674181, MC_U105663148] Funding Source: Medline
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Many copies of mammalian mitochondrial DNA contain a short triple-stranded region, or displacement loop (D-loop), in the major non-coding region. In the 35 years since their discovery, no function has been assigned to mitochondrial D-loops. We purified mitochondrial nucleoprotein complexes from rat liver and identified a previously uncharacterized protein, ATAD3p. Localization studies suggested that human ATAD3 is a component of many, but not all, mitochondrial nucleoids. Gene silencing of ATAD3 by RNA interference altered the structure of mitochondrial nucleoids and led to the dissociation of mitochondrial DNA fragments held together by protein, specifically, ones containing the D-loop region. In vitro, a recombinant fragment of ATAD3p bound to supercolled DNA molecules that contained a synthetic D-loop, with a marked preference over partially relaxed molecules with a D-loop or supercoiled DNA circles. These results suggest that mitochondrial D-loops serve to recruit ATAD3p for the purpose of forming or segregating mitochondrial nucleoids.
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