Journal
ANALYTICAL CHEMISTRY
Volume 79, Issue 2, Pages 540-545Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac061603h
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- NCRR NIH HHS [RR18522] Funding Source: Medline
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Silica-based monolithic capillary columns (25 cm x 10 mu m i.d.) with integrated nanoESI emitters have been developed to provide high-quality and robust microSPE-nanoLC-ESI-MS analyses. The integrated nanoESI emitter adds no dead volume to the LC separation, allowing stable electrospray operation at flow rates of similar to 10 nL/min. In an initial application with a linear ion trap MS, we identified 5510 unique peptides that covered 1443 distinct Shewanella oneidensis proteins from a 300-ng tryptic digest sample in a single 4-h LC-MS/MS analysis. The use of an integrated monolithic ESI emitter provided enhanced resistance to clogging and provided good run-to-run reproducibility.
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