Journal
TALANTA
Volume 71, Issue 1, Pages 123-128Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2006.03.030
Keywords
Herring Sperm DNA (DNA); Basic Brown G (BBG); electrochemical probe; interaction; determination
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A new method of electrochemical probe has been proposed for the determination of Herring Sperm DNA (DNA) based on its interaction with Basic Brown G (BBG). The electrochemical behavior of interaction of BBG with DNA was investigated on Hg electrode. In 0.1 mol L-1 NH3-NH4Cl buffer solution (pH 8.0), BBG can be reduced on Hg electrode with a well-defined voltammettic peak at -0.67 V (versus SCE). In the presence of DNA, the reduction peak current of BBG decreases and the peak potential shifts to a more positive potential without the appearance of new peak. The study shows that a new BBG-DNA complex is formed by linear sweep voltammetry (LSV) and spectrophotometry. The decrease of the second order derivative of reductive peak current (Delta i(p)) of BBG is proportional to the concentration of DNA in the range of 0.10-36 mu g mL(-1). Limit of detection of DNA is 0.04 mu g mL(-1). DNA of Hepatitis B Virus in serum samples was determined satisfactorily. Additionally, the binding mechanism was preliminarily discussed. The mode of interaction between BBG and DNA was found to be intercalation binding. (c) 2006 Elsevier B.V. All rights reserved.
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