Journal
FEBS LETTERS
Volume 581, Issue 2, Pages 279-283Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2006.12.026
Keywords
GcpE; 2-C-methyl-D-erythritol-2,4-cyclodiphosphate; [4Fe-4S]; EPR spectroscopy; resonance Raman spectroscopy; isoprene synthesis
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The GcpE enzyme converts 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (MEcPP) into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) in the penultimate step of the DOXP pathway for isoprene biosynthesis. Purification of the enzyme under exclusion of air leads to a preparation that contains solely [4Fe-4S] clusters. Kinetic studies showed that in the presence of the artificial reductant dithionite and MEcPP a new transient iron-sulfur-based signal is detected in electron paramagnetic resonance (EPR) spectroscopy. Similarity of this EPR signal to that detected in ferredoxin:thioredoxin reductase indicates that during the reaction an intermediate is directly bound to the active-site cluster. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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