Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1140, Issue 1-2, Pages 107-111Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.11.057
Keywords
high-speed counter-current chromatography; polyphenol; 3,4-dihydroxyphenyllactic acid; salvianolic acid B; protocatechualdehyde
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Three kinds of polyphenols of Salvia miltiorrhiza Bunge, 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde, were separated and purified in one step with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:6:1.5:1.5:8) by high-speed countercurrent chromatography. Acetic acid was successfully used to increase the partition of high polar target compounds in organic phase to modify partition coefficient value. 3,4-Dihydroxyphenyl lactic acid, salvianolic acid B and protocatechualdehyde were purified from 100 mg water extracted crude sample of Salvia miltiorrhiza Bunge at purity of 97.6%, 94.2% and 98.2% and at yield of 98.6%, 73.6% and 90.2%. High-speed countercurrent chromatography together with organic/aqueous solvent system supplied an efficient method to purify water-soluble compounds directly from crude samples of traditional Chinese medicines. (c) 2006 Elsevier B.V All rights reserved.
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