Host immune competence and local ischemia affects the functionality of engineered vasculature

Objective: Localized and sustained delivery of vascular endothelial growth factor (VEGF) is a promising approach to overcome the limited efficacy of bolus delivery. The authors examined the effects of host immune competence and local ischemia on the functionality of new vessel networks formed with this approach. Methods: Vessel structure and perfusion resulting front implantation of porous 85:15 poly(lactide-co-glycolide) scaffolds releasing VEGF(165) were measured in both subcutaneous tissue and ischemic hindlimbs of immune competent C57BL/6 and immune deficient: SCID mice. Results: Sustained VEGF delivery resulted in a similar similar to 100% increase in vessel density within Scaffolds in both implant sites; and both animal models. However, the resulting perfusion within scaffolds implanted in subcutaneous tissue increased modestly versus control (18-35%), while perfusion increased 52-110% above control when VEGF-releasing scaffolds were placed in ischemic hindlimbs of C57BL/6 or SCID mice. VEGF delivery unproved perfusion in the entire ischemic limb (55 +/- 18% of die normal value by week 6; 138% increase over control) in SCID mice. Although C57BL/6 mice demonstrated spontaneous recovery front ischemia, VEGF delivery accelerated recovery as compared to control. Conclusions: Localized and sustained VEGF delivery can create functional vasculature that: amplifies recovers of tissue ischemia. However; increases in local and regional perfusion were highly dependent on the implantation site and the animal model.