Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 88, Issue 2, Pages 185-191Publisher
SPRINGER
DOI: 10.1007/s11240-006-9188-x
Keywords
airlift bioreactor; liquid culture; micropropagation; cytokinin; wild clones
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Cultures of three cloudberry (Rubus chamaemorus L.) clones collected from natural stands in Newfoundland and Labrador, Canada were established in vitro on a modified cranberry (Vaccinium macrocarpon Ait.) tissue culture medium containing 8.9 mu M 6-benzylaminopurine (BAP). Clones were compared for in vitro shoot proliferation on gelled medium supplemented with varying levels of BAP and thidiazuron (TDZ). Addition of 5.8 mu M gibberellic acid (GA(3)) in 8.9 mu M BAP-contained medium improved shoot proliferation. TDZ supported rapid shoot proliferation at low concentration (1.1 mu M) but induced 20-30% hyperhydricity in a plastic airlift bioreactor system containing liquid medium. Bioreactor-multiplied hyperhydric shoots were transferred to gelled medium containing 8.9 mu M BAP and 5.8 mu M GA(3) and produced normal shoots within 4 weeks of culture. Genotypes differed significantly with respect to multiplication rate with 'C1' producing the most shoots per explant. Proliferated shoots were rooted on a potting medium with 65-75% of survivability of rooted plants. Present results suggested the possibility of large-scale multiplication of cloudberry shoots in bioreactors.
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