Journal
JOURNAL OF IMMUNOLOGY
Volume 178, Issue 3, Pages 1488-1497Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.178.3.1488
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Funding
- NCI NIH HHS [P30 CA008748, P30-CA08748] Funding Source: Medline
- NIAID NIH HHS [AI46202, AI61484, R01 AI046202] Funding Source: Medline
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In the endosomes of APCs, the MHC class H-like molecule H2-M catalyzes the exchange of class H-associated invariant chain peptides (CLIP) for antigenic peptides. H2-O is another class H-like molecule that modulates the peptide exchange activity of H2-M. Although the expression pattern of H2-O in mice has not been fully evaluated, H2-O is expressed by thymic epithelial cells, B cells, and dendritic cells (DCs). In this study, we investigated H2-O, H2-M, and I-A(b)-CLIP expression patterns in B cell subsets during B cell development and activation. H2-O was first detected in the transitional 1 B cell subset and high levels were maintained in marginal zone and follicular B cells. H2-O levels were down-regulated specifically in germinal center B cells. Unexpectedly, we found that mouse B cells may have a pool of H2-O that is not associated with H2-M. Additionally, we further evaluate H2-O and H2-M interactions in mouse DCs, as well as H2-O expression in bone marrow-derived Ms. We also evaluated H2-O, H2-M, I-A(b), and I-A(b)-CLIP expression in splenic DC subsets, in which H2-O expression levels varied among the splenic DC subsets. Although it has previously been shown that H2-O modifies the peptide repertoire, H2-O expression did not alter DC presentation of a number of endogenous and exogenous Ags. Our further characterization of H2-O expression in DCs, as well as the identification of a potential free pool of H2-O in mouse splenic B cells, suggest that H2-O may have a yet to be elucidated role in immune responses.
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