Journal
EXPERT REVIEW OF PROTEOMICS
Volume 4, Issue 1, Pages 67-78Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1586/14789450.4.1.67
Keywords
automation; baculovirus; cell-free transcription; cell-free translation; Escherichia coli; mammalian; recombinant protein expression
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The expression of high levels of stable and functional proteins remains a bottleneck in many scientific endeavors, including the determination of structures in a high-throughput fashion or the screening for novel active compounds in modern drug discovery. Recently, numerous developments have been made to improve the production of soluble and active proteins in heterologous expression systems. These include modifications to the expression constructs, the introduction of new and/or improved pro- and eukaryotic expression systems, and the development of improved cell-free protein synthesis systems. The introduction of robotics has enabled a massive parallelization of expression experiments, thereby vastly increasing the throughput and, hopefully, the output of such experiments. In addition, the big challenges of recombinant overexpression of membrane and secreted proteins are tackled, and some new methods are reviewed.
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