Journal
CYTOMETRY PART A
Volume 71A, Issue 2, Pages 80-86Publisher
WILEY
DOI: 10.1002/cyto.a.20362
Keywords
laser scanning cytometry; apoptosis; mitochondria; multi-parameter; 2-mercaptopyridine-N-oxide-hydrate sodium salt; cancer cells
Categories
Ask authors/readers for more resources
Background: Simultaneous analysis of multiple intracellular events is critical for assessing the effect of biological response modifiers, including the efficacy of chemotherapy. Here we used the automated laser scanning cytometry (LSC) for multi-parameter analysis of drug-induced tumor cell apoptosis. Materials: Using 2-mercaptopyridine-N-oxide-hydrate sodium salt, or the commonly used chemotherapeutic agents etoposide and camptothecin, we performed simultaneous analyses of apoptosis-related morphological features as well as fluorescence-based biochemical changes in a 96-well format. Results: We demonstrate the scope of LSC as a platform for comparing multiple variables between different cell populations, distinguishing unique events at a single cell level within a sample population, and enabling simultaneous screenings in a single assay at multiple dosages and time-points. Conclusion: These data underscore the power of LSC for simultaneous multi-parameter analysis, which could have implications for screening or assessing the efficacy of drug responses in heterogeneous cell populations and at the single cell level. (c) 2007 International Society for Analytical Cytology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available