Journal
BIOLOGICAL CHEMISTRY
Volume 388, Issue 2, Pages 181-183Publisher
WALTER DE GRUYTER & CO
DOI: 10.1515/BC.2007.020
Keywords
fluorescence titration; peptide binding; protein conformation
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HIV-1 Nef is a small protein (approx. 25 kDa) that is post-translationally modified by myristoylation. To explain its complex activities, a 'Nef-cycle' is discussed, which postulates different molecular conformations of Nef. Using recombinant full-length non-myristoylated Nef and synthetic peptides, we demonstrate by fluorescence titration experiments that a peptide representing the myristoylated N-terminus of Nef is specifically bound by Nef. A non-myristoylated N-terminal fragment of Nef or a myristoylated control peptide does not bind to Nef. These results are the first direct experimental evidence of the existence of a myristate-binding pocket in Nef, a prerequisite of the postulated 'closed' Nef conformation.
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