Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 68, Issue 2, Pages 434-436Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2006.09.009
Keywords
DNA libraries; metagenomics; screening
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A semiliquid medium was employed in an efficient method to screen highly complex DNA libraries for clones with known sequences. Unbiased clone pools are generated in 2 ml vials and screened by whole-cell PCR, and individual clones are obtained by few additional rounds of dilution and PCR screening. To demonstrate the utility of this approach, the single positive clone present in a 400,000 member metagenomic fosmid library was isolated. (c) 2006 Elsevier B.V. All rights reserved.
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