Journal
JOURNAL OF CELL BIOLOGY
Volume 176, Issue 5, Pages 653-665Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200608041
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Funding
- NIDDK NIH HHS [P30 DK032520, P30 DK32520] Funding Source: Medline
- NIGMS NIH HHS [GM14642, R01 GM060992, GM60992, R37 GM014642, R01 GM014642, R37 GM030626] Funding Source: Medline
- PHS HHS [G30626] Funding Source: Medline
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Intraflagellar transport (IFT), which is the bidirectional movement of particles within flagella, is required for flagellar assembly. IFT particles are composed of similar to 16 proteins, which are organized into complexes A and B. We have cloned Chlamydomonas reinhardtii and mouse IFT46, and show that IFT46 is a highly conserved complex B protein in both organisms. A C. reinhardtii insertional mutant null for IFT46 has short, paralyzed flagella lacking dynein arms and with central pair defects. The mutant has greatly reduced levels of most complex B proteins, indicating that IFT46 is necessary for complex B stability. A partial suppressor mutation restores flagellar length to the ift46 mutant. IFT46 is still absent, but levels of the other IFT particle proteins are largely restored, indicating that complex B is stabilized in the suppressed strain. Axonemal ultrastructure is restored, except that the outer arms are still missing, although outer arm subunits are present in the cytoplasm. Thus, IFT46 is specifically required for transporting outer arms into the flagellum.
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