Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 55, Issue 2, Pages 111-118Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/jhc.6A7024.2006
Keywords
diabetes; islets; islet amyloid polypeptide; morphometry; immunohistochemistry
Categories
Funding
- NCI NIH HHS [CA-367127] Funding Source: Medline
Ask authors/readers for more resources
There is a lack of agreement on the distribution of islet amyloid polypeptide (IAPP) in the pancreases of healthy and diabetic subjects. Therefore, a detailed morphometrical and immunohistochemical study was performed to obtain information on the distribution of cells expressing insulin, glucagon, somatostatin, pancreatic polypeptide (PP), and IAPP in the pancreases of non-diabetic (n=4) and diabetic individuals (n=6). In the non-diabetic cases, P-cells contributed to similar to 64%, alpha-cells to 26%, delta-cells to 8%, PP cells to 0.3%, and IAPP cells to 34% of the islet cell population. The ratio of IAPP/insulin was similar to 1:2. In diabetic cases, P-cells were decreased by 24%, and IAPP was decreased by 57%. The alpha- and delta-cells were increased by 40% and 58%, respectively. IAPP/insulin ratio was decreased by 41%. Thus, only 50% of the beta-cells in non-diabetics and only 30% in diabetics coexpressed IAPP. In diabetics, more delta-cells coexpressed IAPP than in non-diabetics. The results seem to argue against the notion that the secretion of IAPP is increased in diabetics. It is possible that an increase in somatostatin and glucagon plays a greater role in diabetes than IAPP.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available