Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 71, Issue 2, Pages 598-602Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.60536
Keywords
Rooibos tea; HP20 column; immunoglobulin M (IgM); interleukin-10 (IL-10)
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Rooibos tea was extracted with boiling water. The aqueous extract was chromatographed in a Diaion HP20 column eluted stepwise with water, 25%, 50% and 75% (v/v) aqueous methanol, and 100% methanol. The water eluate (fraction A) showed an augmenting effect on anti-ovalbumin (anti-OVA) immunoglobulin M (IgM) production in OVA-stimulated murine splenocytes in vitro. Fraction A also showed a strong augmenting effect on interleukin-10 generation in murine splenocytes. Furthermore, continuous ingestion of fraction A was found to increase the anti-OVA IgM level in the sera of OVA-immunized mice.
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