Journal
CLINICA CHIMICA ACTA
Volume 376, Issue 1-2, Pages 136-141Publisher
ELSEVIER
DOI: 10.1016/j.cca.2006.08.005
Keywords
micrococcus lysodeikticus; lysozyme activity; resonance scattering spectral assay
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Background: Several methods, including turbidimetric and colorimetric methods, have been reported for the detection of lysozyme activity. However, there is no report about the resonance scattering spectral (RSS) assay, which is based on the catalytic effect of lysozyme on the hydrolysis of micrococcus lysodeikticus (ML) and its resonance scattering effect. Methods: Results: ML has 5 resonance scattering peaks at 360 400, 420, 470, and 520 nm with the strongest one at 470 nm. The concentration of ML in the range of 2.0 x 10(6) x 9.3 x 10(8) cells/ml is proportional to the RS intensity at 470 nm (1470). A new catalytic RSS method has been proposed for 0.24-40.0 U/ml (or 0.012-2.0 mu g/ml) lysozyme activity, with a detection limit (3 sigma) of 0.014 U/ml (or 0.0007 mu g/ml). Saliva samples were assayed by this method, and it is in agreement with the results of turbidimetric method. The slope, intercept and the correlation coefficient of the regression analysis of the 2 assays were 0.9665, -87.50, and 0.9973, respectively. Conclusion: The assay has high sensitivity and simplicity. (c) 2006 Elsevier B.V. All rights reserved.
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