Journal
PLANTA MEDICA
Volume 73, Issue 2, Pages 157-162Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/s-2007-967106
Keywords
oleanolic acid; betulinic acid; solubility; synergy; Loranthaceae; Viscum album L; aqueous mistletoe extracts
Ask authors/readers for more resources
Mistletoe (Viscum album L.) contains the triterpene acids oleanolic acid (OA) and betulinic acid (BA), which were found to have anti-tumour properties. In this study, the solubilities of OA and BA were studied in water (up to 0.02 mu g/mL in each case) and in alkaline solutions of 10 mM trisodium phosphate (pH 11.5; OA: 77.2 mu g/mL; BA: 40.1 mu g/mL). Furthermore, triterpene acids were quantified in aqueous mistletoe extracts (pH 7.3; drug to extract ratio 1 :25). OA (1.1 mu g/mL) and BA (0.9 mu g/mL) were extracted with a yield of less than 5%. Preparing plant extracts with basic pH values resulted in a triterpene acid content of 9.3 mu g/mL OA and 5.2 mu g/mL BA (pH 12.1), reaching neither the solubility limits nor a complete extraction of the plant material. The triterpene acid content of neutral plant extracts above the solubility limit could be due to interactions with biocolloids. Interaction studies were performed by gel permeation chromatography. Different mechanisms of the dissolution at pH 7.3 and pH 10.2 are discussed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available