Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 45, Issue 2, Pages 317-323Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01549-06
Keywords
-
Categories
Funding
- Wellcome Trust [074898, 069615] Funding Source: Medline
Ask authors/readers for more resources
A multilocus sequence typing (MLST) scheme for Candida krusei was devised, based on sequencing of six gene fragments of the species. The existence of heterozygous results for each of the six fragments sequenced confirms that C krusei is diploid for at least part of its genome. The C krusei MLST scheme had a discriminatory index of 0.998, making this system ideal for strain typing of C. krusei clinical isolates. MLST data for 122 independent C. krusei isolates from a range of geographical sources were analyzed by eBURST, structure, and the unweighted-pair group method using average linkages to derive a population structure comprising four subtype strain clusters. There was no evidence of geographical associations with particular subtypes. Data for pairs of isolates from seven patients showed that each patient was colonized and/or infected with strain types that were indistinguishable by MLST. The C krusei MLST database can be accessed online at http://pubmlst.org/ckrusei.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available