Journal
LEUKEMIA
Volume 21, Issue 2, Pages 297-303Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.leu.2404470
Keywords
VSEL; cord blood; SSEA-4; Oct-4; CXCR4; CD133
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Funding
- NCI NIH HHS [R01 CA106281-01] Funding Source: Medline
- NIDDK NIH HHS [R01 DK074720] Funding Source: Medline
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Recently, we purified from adult murine bone marrow ( BM) a population of CXCR4(+), Oct-4(+) SSEA-1+, Sca-1(+) lin(-) CD45(-) very small embryonic-like (VSEL) stem cells and hypothesized that similar cells could be also present in human cord blood ( CB). Here, we report that by employing a novel two-step isolation procedure-removal of erythrocytes by hypotonic lysis combined with multiparameter sorting-we could isolate from CB a population of human cells that are similar to murine BM-derived VSELs, described previously by us. These CB-isolated VSELs (CB-VSEL)are very small (3-5 mu m) and highly enriched in a population of CXCR4(+) AC133(+) CD34(+) lin(-) CD45(-) CB mononuclear cells, possess large nuclei containing unorganized euchromatin and express nuclear embryonic transcription factors Oct-4 and Nanog and surface embryonic antigen SSEA-4. Further studies are needed to see if human CB-isolated VSELs similar to their murine BM-derived counterparts are endowed with pluripotent stem cell properties.
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